Na+H+ exchange in rabbit ileal brush border membranes
نویسندگان
چکیده
منابع مشابه
Oxalate transport by anion exchange across rabbit ileal brush border.
This study demonstrates the presence of oxalate transporters on the brush border membrane of rabbit ileum. We found that an inside alkaline (pH = 8.5 inside, 6.5 outside) pH gradient stimulated [14C]oxalate uptake 10-fold at 1 min with a fourfold accumulation above equilibrated uptake at 5 min. 1 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonate (disodium salt; DIDS) profoundly inhibited the pH-g...
متن کاملQuantitative contribution of NHE2 and NHE3 to rabbit ileal brush-border Na+/H+exchange.
Intestinal neutral NaCl absorption, which is made up of brush-border (BB) Na+/H+exchange linked to BB Cl-/[Formula: see text]exchange, is up- and downregulated as part of digestion and diarrheal diseases. Glucocorticoids stimulate ileal NaCl absorption and BB Na+/H+exchange. Intestinal BB contains two Na+/H+exchanger isoforms, NHE2 and NHE3, but their relative roles in rabbit ileal BB Na+/H+exc...
متن کاملCalcium/calmodulin inhibition of coupled NaCl transport in membrane vesicles from rabbit ileal brush border.
The role of Ca2+ and calmodulin in regulating coupled NaCl transport has been investigated in membrane vesicles from rabbit ileal brush border. Uptake of 22Na+ and 36Cl- was determined by a rapid filtration technique in vesicles isolated with a sucrose density gradient ultracentrifugation method. Ca2+ on the inside of the vesicle inhibited Na+ uptake when Cl- was the anion and Cl- uptake when N...
متن کاملConductive pathways for chloride and oxalate in rabbit ileal brush-border membrane vesicles.
To evaluate the possibility that an apical membrane conductive pathway for oxalate is present in the rabbit distal ileum, we studied oxalate ([14C]oxalate) and chloride (36Cl) uptake into brush-border membrane vesicles enriched 15- to 18-fold in sucrase activity. Voltage-sensitive pathways for oxalate and chloride were identified by the stimulation of uptake provided by an inwardly directed pot...
متن کاملBrush Border Membranes. Ciba Foundation Symposium 95
paradox tacitly describes the specific and dynamic pathways along which plasma membranes must be moved in cells, and several studies on the nature and regulation of this complex system have been assembled and summarized in this volume, which contains papers and discussions from a symposium on membrane recycling held at the Ciba Foundation in London in January 1982. As is the case with nearly al...
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ژورنال
عنوان ژورنال: Gastroenterology
سال: 1984
ISSN: 0016-5085
DOI: 10.1016/0016-5085(84)90213-0